Description
Glutathione S-Transferase (GST) from Schistosoma japonicum is a 26 kD protein that has become a popular fusion partner for high expression of recombinant proteins. GST tags allow recombinant fusion proteins to be purified in a single nondenaturing affinity step on glutathione-coupled beads. The high affinity of the GST-glutathione interaction results in simple near-homogeneous purifications. Clone G172-1138 recognizes GST. A recombinant GST-tagged protein was used as immunogen.
Format
- FormatPurified
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Applications include western blot analysis (1-2 µg/ml antibody). The antibody is useful in screening for recombinant GST-tagged proteins produced in bacteria or in insect cells infrected with recombinant baculovirus.
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