Description
CD33, clone P67.6, is derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with FMY9S5 cells containing the CD33 gene.
CD33 recognizes a human myelomonocytic antigen, Mr 67 kilodaltons (kd).
Format
- FormatAPC
- Excitation SourceRed 633 nm
- Excitation Max650nm
- Emission Max660nm
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
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Preparation and Storage
The monoclonal antibody is supplied as 12 μg purified immunoglobulin in 2.0 mL (6 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 3 μg in 1.0 mL (3 μg/mL) of PBS. The PE conjugate is supplied as 24 μg in 2.0 mL (12 μg/mL) of PBS. The PerCP-Cy5.5 conjugate is supplied as 6.3 μg in 1.0 mL (6.3 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 12.5 μg in 0.5 mL (25 μg/mL) of PBS. The APC conjugate is supplied as 6.2 μg in 0.5 mL (12.4 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.
Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
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