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主营:医疗设备、医疗耗材、实验仪器、抗体、试剂、诊断等产品
℡ 4000-520-616
℡ 4000-520-616
BD Biosciences/647 EdU Click Proliferation Kit/50Tests/565456
产品编号:565456
市  场 价:¥377832.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
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美  元  价:$17992.00
品      牌: BD Biosciences
公      司:BD Biosciences,Inc.
公司分类:
BD Biosciences/647 EdU Click Proliferation Kit/50Tests/565456
商品介绍
Product DetailsRecommended AssayReferences

Description

The BD Pharmingen™ 647 EdU Click Proliferation Kit provides a tool for analyzing DNA synthesis in cycling cells. In this method, the thymidine analog EdU (5-ethynyl-2´-deoxyuridine) is incorporated into newly synthesized DNA by cells progressing through the S (DNA synthesis) phase of the cell cycle. EdU can then be detected in fixed and permeabilized cells with a fluorophore-labeled azide. The reaction of the fluorophore-labeled azide and EdU occurs via a copper-catalyzed click reaction between the azide moiety of the fluorophore and the alkyne moiety of EdU. When co-stained with a DNA dye such as 7-AAD, PI, or DAPI, cell populations may be segmented by flow cytometry into the G0/G1-phases (2N DNA content, EdU-negative), S-phase (2N-4N DNA content, EdU-positive), or G2/M-phases (4N DNA content, EdU-negative).

The BD Pharmingen™ 647 EdU Click Proliferation Kit containsEterneon™ Red 645 Azide, which is excited by the red laser and has an excitation maximum of 643 nm and an emission maximum of 662 nm.

Please note that this kit is provided as Part 1 of 2 (Components A, B, and C) to be stored dry and protected from light at -20°C, and Part 2 of 2 (Components D, E, F, and G) to be stored dry at 2 - 8°C or room temperature. See "Kit Contents" immediately below for more information.

Kit Contents

Component IDComponent DescriptionAmountLong Term Storage Conditions

AEdU (5-ethynyl-2´-deoxyuridine)10 mg -20°C, dry

BEterneon™ Red 645 azide (10 mM)130 μL -20°C, dry, dark

CBuffer Additive (10×)400 mg -20°C, dry

DSaponin-based Permeabilization and Wash Reagent (10×)50 mL2 - 8°C

EFixative Solution (4% paraformaldehyde-based)5 mL2 - 8°C

FCatalyst Solution2 mL RT, dry

GDMSO5 mL RT, dry

Please note that Hazard Warnings for components listed above are found on Page 5 of this document.

Suggested Companion Products

DAPI Solution RUO 1mgCat No: 564907

Alexa Fluor® 488 Rat anti-Histone H3 (pS28) HTA28RUO 100TestsCat No: 558610

Stain Buffer (FBS) RUO 500mLCat No: 554656

Stain Buffer (BSA) RUO 500mLCat No: 554657

PI/RNase Staining Buffer RUO 100mLCat No: 550825

Propidium Iodide Staining Solution RUO 2mLCat No: 556463

7-AAD RUO 2mLCat No: 559925

Product Notices

  1. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Eterneon™ is a trademark of baseclick GmbH
  5. Limited Use Label License — Research Use Only: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product only to perform research, and not for any clinical, diagnostic, vaccine, protective, prophylactic, preventive or therapeutic use in humans, animals, or plants.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. This product is provided under an intellectual property license from Life Technologies Corporation.The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or(d) resale, whether or not resold for use in research.For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  1. Buck SD, Bradford J, Gee KR, Agnew BJ, Clarke ST, Salic A. Detection of S-phase cell cycle progression using 5-ethynyl-2’-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2’-deoxyuridine antibodies. Biotechniques. 2008; 44(7):927-929.View reference

  2. Cavanagh BL, Walker T, Norazit A, Meedeniya AC. Thymidine analogues for tracking DNA synthesis. Molecules. 2011; 16(9):7980-7993.View reference

  3. Kolb HC, Finn MG, Sharpless KB. Click Chemistry: Diverse Chemical Function from a Few Good Reactions. Angew Chem Int Ed Engl. 2001; 40(11):2004-2021.View reference

  4. Kotogany E, Dudits D, Horvath GV, Ayaydin F. A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine. Plant Methods. 2010; 6(1).View reference

  5. Salic A, Mitchison TJ. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci U S A. 2008; 105(7):2415-2420.View reference

  6. Wang Q, Chan TR, Hilgraf R, Fokin VV, Sharpless KB, Finn MG. Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition. J Am Chem Soc. 2003; 125(11):3192-3193.View reference

品牌介绍

BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗,提高传染性疾病诊断的质量和速度,推进新型药物和疫苗的研究与发现。公司于1897年在纽约成立,总部位于美国新泽西州的富兰克林湖,业务可分为BD医疗、BD诊断、BD生物科学三大类,生产销售包括医用耗材、实验室仪器、抗体、试剂、诊断等产品。

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