商品介绍
Product DetailsRecommended AssayReferences
Description
The Jurkat Apoptotic Lysate Set I is designed to be used as a western blot positive control for detecting antigens expressed during apoptosis. Jurkat cells in log-phase growth were either left untreated or treated with 4 µM camptothecin for 4 hours to induce apoptosis. The cells were collected, lysed in lysis buffer [1% SDS, 1 mM sodium orthovanadate, 10 mM Tris (pH 8.0)], and aliquoted in SDS sample buffer.
Components
| Part No. | Description | Size |
|---|---|---|
| 51-16506Y | Jurkat Control Lysate | 500µg (1 ea) |
| 51-16606Y | Camptothecin Treated Jurkat Lysate | 500µg (1 ea) |
| Resources & Tools | ||||||
|---|---|---|---|---|---|---|
| SpectrumViewer | Download TDS | Regulatory Document Website | ||||
Preparation and Storage
Store undiluted at -20°C.Do not dilute or boil.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Western blot:Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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