CD57, clone HNK-1, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with membrane extracts of the HSB-2 T–lymphoblastoid cell line.
CD57 (Leu-7) recognizes an Mr 110-kdalton (kDa) glycoprotein that is expressed on a subset of natural killer (NK) lymphocytes and T lymphocytes. The antigen is a carbohydrate structure associated with myelin-associated glycoprotein (MAG).
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The monoclonal antibody is supplied as 25 µg purified immunoglobulin in 2.0 mL (12.5 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 25 µg in 2.0 mL (12.5 µg/mL). The biotin conjugate is supplied as 25 µg in 2.0 mL (12.5 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
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