Description
Interleukin-1β (IL-1β) is a potent immunomodulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. This is the mature form of human IL-1β, a 17 kD protein containing 153 amino acid residues, representing amino acids A117 through S269 of the IL-1β precursor protein, expressed in E. coli.Recombinant human IL-1β (Cat. No. 554602) is lyophilized from a solution comprised of 0.22 μm sterile-filtered aqueous buffered solution with no preservatives.Recombinant human IL-1β is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 1.0 EU per µg of human IL-1β as measured in a chromogenic LAL assay.
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Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions.This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Recombinant human IL-1β (Cat. No. 554602) may be reconstituted in sterile neutral buffer containing 0.5 - 10 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at – 80°C.For in vitro biological assay use, carrier-protein concentrations of 0.5 - 1 mg/mL are recommended.For use as an ELISA standard, carrier-protein concentrations of 5 - 10 mg/mL are recommended.Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.Carrier proteins should be pre-screened for possible effects in each investigator"s experimental system.Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
Bioassay:Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments.An activity range of 1.0 - 4.0 x 10^8 units/mg, encompassing an
ED50= 25 - 100 pg/mL, has previously been reported using TF-1 as indicator cells for proliferaiton, with a unit defined asthe amount of material needed to stimulate a half-maximal response at cytokine saturation.
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