Description

Programmed cell death (apoptosis) may be induced in response to a variety ofcytotoxic stimuli, including activation of surface receptors. An area of particular interest has been the induction of apoptosis by the receptor-ligand pair: Fas (CD95) and Fas Ligand (FasL). Fas is an ~45 kD cell surface protein whichbelongs to the the TNF (tumor necrosis factor receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL, a member of the TNF ligand family, is expressed on activated T and NK cells. FasL initiates signaling at the cell surface by aggregation of individual Fas recpetors via binding to the multivalent ligand. Antibodies which selectively activate Fas can be used in vitro to mimic the apoptotic response associated with FasL.Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development.Clone G254-274 recognizes human Fas. A soluble form of recombinant human Fas (lacking the Fas transmembrane domain) was used as immunogen.

Format

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Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.

Product Notices

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.