CD19, clone 4G7, is derived from hybridization of mouse P3-X63-Ag8.653 cells with spleen cells from BALB/c mice immunized with human chronic lymphocytic leukemia (CLL) cells.
CD19 (Leu-12) recognizes a human B-lymphocyte antigen, Mr 90 kdaltons (kDa).
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The monoclonal antibody is supplied as 50 μg purified immunoglobulin in 2.0 mL (25 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 50 μg in 2.0 mL (25 μg/mL). The PE conjugate is supplied as 25 μg/mL in 1.0 mL. The PerCP conjugate is supplied as 12.5 μg/mL in 2.0 mL (6.25 μg/mL). PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
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