Description
Anti–P-glycoprotein (P-gp), clone 15D3, is generated from the fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with a BA3T3 fibroblast line (BATV.2) transfected with the human MDR1 gene.
Anti–P-glycoprotein (P-gp) recognizes a 170-kdalton (kDa) protein from multidrug resistant (MDR) cells.
Format
- FormatPE
- Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max496nm
- Emission Max578nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
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Preparation and Storage
The PE conjugate is supplied in 1.0 mL of PBS. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
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