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℡ 4000-520-616
℡ 4000-520-616
BD Biosciences/Purified Mouse Anti-Human TCR-α/β/20 μL/347770
产品编号:347770
市  场 价:¥6005.16
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
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美  元  价:$285.96
品      牌: BD Biosciences
公      司:BD Biosciences,Inc.
公司分类:
BD Biosciences/Purified Mouse Anti-Human TCR-α/β/20 μL/347770
商品介绍
Product DetailsRecommended AssayReferences

Description

Clone WT31 is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

WT31 (Anti–TCR-α/β-1) recognizes a conformational epitope formed by the T-cell receptor (TCR) for antigen and the CD3 epsilon chain. The α/β TCR is a disulfide-linked 80-kdalton (kDa) heterodimer consisting of a 44-kDa α chain and a 37-kDa β chain.

Preparation and Storage

The monoclonal antibody is supplied as 12.5 µg purified immunoglobulin in 2.0 mL (6.25 µg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 100 µg in 2.0 mL (50 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

  1. Brenner M, McClean J, Dialynas D, et al. Identification of a putative second T cell receptor. Nature. 1986;322:145

  2. Gupta S, Shimizu M, Ohira K, Vayuvegula B. T-cell activation via the T-cell receptor: A comparison between WT31 (defining alpha/beta TCR)-induced and anti-CD3-induced activation of human T lymphocytes. Cell Immunol. 1991;132:26-44.

  3. Lanier LL, Weiss A. Presence of Ti (WT31) negative T lymphocytes in peripheral blood and thymus. Nature. 1986;324:268.

  4. Oettgen HC, Kappler J, Tax WJ, Terhorst C. Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J Biol Chem. 1984;259:12039-12048.

  5. Salmerón A, Sanchez-Madrid F, Ursa MA, et al. A conformational epitope expressed upon association of CD3ε with either CD3δ or CD3γ is the main target for recognition by Anti-CD3 monoclonal antibodies. J Immunol. 1991;147:3047-3052.

  6. Spits H, Borst J, Tax WJ, Capel P, Terhorst C, de Vries J. Characteristics of a monoclonal antibody (WT31) that recognizes a common epitope on the human T cell receptor for antigen. J Immunol. 1985;135:1922.

  7. Tax WJ, Leeuwenberg HF, Willems HM, Capel PJ, Koene RA. Monoclonal antibodies reactive with OKT3 antigen of OKT8 antigen. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman S, eds. Leucocyte Typing. Berlin: Springer-Verlag; 1984:721-722.

  8. Tax WJ, Willems HM, Reekers PP, Capel PJ, Koene RA. Polymorphism in mitogenic effect of IgG1 monoclonal antibodies against T3 antigen on human T cells. Nature. 1983;304:445.

  9. Weiss A, Newton M, Crommie D. Expression of T3 in association with a molecule distinct from the T cell antigen receptor heterodimer. Proc Natl Acad Sci USA. 1986;83:6998.

品牌介绍

BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗,提高传染性疾病诊断的质量和速度,推进新型药物和疫苗的研究与发现。公司于1897年在纽约成立,总部位于美国新泽西州的富兰克林湖,业务可分为BD医疗、BD诊断、BD生物科学三大类,生产销售包括医用耗材、实验室仪器、抗体、试剂、诊断等产品。

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