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℡ 4000-520-616
BD Biosciences/CD3 FITC/CD16 + CD56 PE/CD45 PerCP/CD19 APC/20 µL/340500
产品编号：340500
市  场 价：¥0.00
场      地：美国(厂家直采)
产品分类： 蛋白类>多肽>多肽合成>
联系QQ：1570468124
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美  元  价：待定
品      牌： BD Biosciences
公      司：BD Biosciences,Inc.
公司分类：
BD Biosciences/CD3 FITC/CD16 + CD56 PE/CD45 PerCP/CD19 APC/20 µL/340500
商品介绍
Product DetailsRecommended AssayReferences

Description

BD MultiTEST™ CD3 fluorescein isothiocyanate (FITC)/CD16+CD56 phycoerythrin (PE)/CD45 peridinin chlorophyll protein (PerCP)/CD19 allophycocyanin (APC) is a four-color direct immunofluorescence reagent for use with a suitably equipped flow cytometer to identify and determine the percentages and absolute counts of mature human T (CD3+), natural killer (NK) (CD3–CD16+CD56+), and B (CD3–CD19+) lymphocytes in erythrocyte-lysed whole blood. When used with TruCOUNT™ Tubes, absolute counts of these populations can be enumerated from a single tube.

BD MultiTEST reagent and TruCOUNT Tubes can be used with the FACS Loader.BD MultiTEST reagent and TruCOUNT Tubes can be used with the FACS Loader.

Contents

SpecificityCloneFormatIsotypeEntrez Gene ID
CD19SJ25C1 (also known as SJ25-C1)APCIgG1, κ930
CD3SK7 (also known as Leu-4)FITCIgG1, κ916
CD56 (NCAM-1)NCAM16.2 (also known as NCAM 16)PEIgG2b, κ4684
CD16 (FcγRIII)B73.1PEIgG1, κ
CD452D1PerCPIgG1, κ
Resources & Tools
SpectrumViewerDownload MSDSDownload TDS
Preparation and Storage

1. Store the reagent at 2–8°C. Do not use after the expiration date shown on the label.

2. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry.

3. Store Trucount tubes in their original foil pouch at 2–25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch and do not use beyond the expiration date indicated on the packaging.

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  3. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture: Approved Standard. Villanova, PA: National Committee for Clinical Laboratory Standards; 1991. NCCLS document H3-A3.

  4. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline. Villanova, PA: National Committee for Clinical Laboratory Standards; 1991. NCCLS document M29-T2.

  5. Brenner MB, Groh V, Porcelli SA, et al. Structure and distribution of the human γ/δ T-cell receptor. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1986;322:145-149.

  6. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.

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  8. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:788-803.

  9. Cohen S, Weetman A. Activated interstitial and intraepithelial thyroid lymphocytes in autoimmune thyroid disease. Acta Endocr. 1988;119:161-166.

  10. Cunningham BA, Hemperly JJ, Murray BA, Prediger EA, Brackenbury R, Edelman GM. Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing. Science. 1987;236:799-806.

  11. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD19. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.

  12. Fitzgerald-Bocarlsy P, Herberman R, Hercend T, et al. A definition of natural killer cells. In: Ades E, Lopez C, eds. Natural Killer Cells and Host Defense. 82,8756944444444

  13. Foucar K, Goeken J. Clinical application of immunologic techniques to the diagnosis of lymphoproliferative and immunodeficiency disorders. Lab Medicine. 1982;13:403.

  14. Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:236-246.

  15. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:3-30.

  16. Jackson AL, Warner NL. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose NR, Friedman H, Fahey JL, eds. Manual of Clinical Laboratory Immunology. 3rd ed. Washington, DC: American Society for Microbiology; 1986:226-235.

  17. Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983;131:536-539.

  18. Knowles RW. Immunochemical analysis of the T-cell– specific antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:259- 288.

  19. Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH. Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J. Immunol. 1991;146:4421-4426.

  20. Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH- 1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986;136:4480-4486.

  21. Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human B Lymphocytes. 1986;2:3-43.

  22. Nicholson J, Kidd P, Mandy F, Livnat D, Kagan J. Three-color supplement to the NIAID DAIDS guideline for flow cytometric immunophenotyping. Cytometry. 1996;26:227- 230.

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  24. Nicholson JK, Browning SW, Orloff SL, McDougal JS. Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry. J Immunol Methods. 1993;160:215-218.

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  33. Schmidt RE. Non-lineage/natural killer section report: new and previously defined clusters. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Osford University Press; 1989:517-542.

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