BD Biosciences/Purified Mouse Anti-Human CD34/20 μL/347660,BD Biosciences,,BD Biosciences,bdbiosciences,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：BD Biosciences/Purified Mouse Anti-Human CD34/20 μL/347660

产品编号：347660

市场价：¥6005.16

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$285.96

品牌： BD Biosciences

公司：BD Biosciences,Inc.

公司分类：

商品介绍

Product DetailsRecommended AssayReferences

Description

CD34 (Anti–HPCA-1), clone My10, is derived from the hybridization of mouse Sp2/0-Ag14 cells with spleen cells of BALB/c mice immunized with the human cell line KG-1a.

CD34 (Anti–HPCA-2), clone 8G12, is derived from the hybridization of mouse Sp2/0-Ag14 cells with spleen cells of BALB/c mice immunized with the human cell line KG-1a.

The CD34 antigen is a single-chain transmembrane glycoprotein, Mr 105 to 120 kilodaltons (kd). The antigen is associated with human hematopoietic progenitor cells and is a differentiation stage–specific leucocyte antigen. Clone My10 and clone 8G12 recognize two distinct CD34 epitopes; at least three epitopes have been identified.

Format

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Preparation and Storage

Clone My10: The monoclonal antibody is supplied as 100 µg purified immunoglobulin in 2.0 mL (50 µg/mL) of phosphate-buffered saline (PBS).

Clone 8G12: The monoclonal antibody is supplied as 50 µg purified immunoglobulin in 2.0 mL (25 µg/mL) of PBS. The FITC and PE conjugates are each supplied as 50 µg in 2.0 mL (25 µg/mL) of PBS. The PerCP conjugate is supplied as 50 µg in 1.0 mL (50 µg/mL) of PBS. The PerCP-Cy5.5 conjugate is supplied as 12.5 µg in 1.0 mL (12.5 µg/mL) of PBS. The PE-Cy7 conjugate is supplied as 25 µg in 0.5 mL (50 µg/mL) of PBS. The APC conjugate is supplied as 50 µg in 0.5 mL (100 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide. Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

Andrews RG, Singer JW, Bernstein ID. Precursors of colony-forming cells in humans can be distinguished from colony-forming cells by expression of the CD33 and CD34 antigens and light scattering properties. J Exp Med. 1989;169:1721-1731.
Brocklebank AM, Sparrow RL. Enumeration of CD34⁺ cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy. Cytometry. 2001;46:254-261.
Civin C, Banquerigo M, Strauss L, Loken MR. Antigenic analysis of hematopoiesis. VI. Characterization of MY-10-positive progenitor cells in normal human bone marrow. Exp Hematol. 1987;15:10-17.
Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH. Antigenic analysis of hematopoiesis. III. A hemopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol. 1984;133:157-165.
Civin CI, Trischmann TM, Fackler MJ, et al. Report on the CD34 cluster workshop. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:818-825.
Gore SD, Kastan MB, Civin CI. Normal human bone marrow precursors that express terminal deoxynucleotidyl transferase include T-cell precursors and possible lymphoid stem cells. Blood. 1991;77:1681-1690.
Greaves MF, Titley I, Colman SM, et al. CD34 cluster workshop report. In: Schlossman SF, Boumsell L, Gilks W, et al, eds. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Osford University Press; 1995;1:840-846.
Hurwitz CA, Loken MR, Graham ML, et al. Asynchronous antigen expression in B lineage acute lymphoblastic leukemia. Blood. 1988;72:299-307.
Kurtzberg J, Denning SM, Nycum LM, Singer KH, Haynes BF. Immature human thymocytes can be driven to differentiate into nonlymphoid lineages by cytokines from thymic epithelial cells. Proc Natl Acad Sci USA. 1989;86:7575-7579.
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Lu L, Walker D, Broxmeyer H, Hoffman R, Hu W, Walker E. Characterization of adult human marrow hematopoietic progenitors is highly enriched by two-color cell sorting with My10 and major histocompatibility class II monoclonal antibodies. J Immunol. 1987;139:1823-1829.
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Siena S, Bregni M, Brando B, et al. Flow cytometry for clinical estimation of circulating hematopoietic progenitors for autologous transplantation in cancer patients. Blood. 1991;77:400-409
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