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℡ 4000-520-616
BD Biosciences/CD3 FITC/20 µL/340542
产品编号:340542
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
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BD Biosciences/CD3 FITC/20 µL/340542
商品介绍
Product DetailsRecommended AssayReferences

Description

Becton Dickinson Immunocytometry Systems (BDIS) CD3 fluorescein isothiocyanate (FITC) reagent is a single-color direct immunofluorescence reagent for identifying and enumerating T lymphocytes using fluorescence microscopy or flow cytometers such as the FACSCalibur™, FACSort™, FACScan™, or FACS Analyzer™.

Preparation and Storage

1. For in vitro diagnostic use.

2. When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.

3. The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.

4. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

5. The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

  1. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline. Villanova, PA: National Committee for Clinical Laboratory Standards; 1991. NCCLS document M29-T2.

  2. Brain P, Gordon J, Willets W. Rosette formation by peripheral lymphocytes. Clin Exp Immunol. 1970;6:681-688.

  3. Brenner M, Groh V, Porcelli S, et al. Structure and distribution of the human γ/δ T-cell receptor. In: Knapp W, Dörken B, Gilks W, et al., eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York: Oxford University Press; 1989:1049-1053.

  4. Brown G, Greaves M. Enumeration of absolute numbers of T and B cells in human blood. Scand J Immunol. 1974;3:161.

  5. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.

  6. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annu Rev Immunol. 1988;6:629-662.

  7. Dwyer J, Finklestein F, Mangi R, Fisher K, Hendler E. Assessment of the adequacy of immunosuppressive therapy using microscopy techniques to study immunologic competence. Transplant Proc. 1975;7:785.

  8. Freidrich W, O"Reilly R, Kozenger B, Gebhard D, Good R, Evans R. T lymphocyte reconstitution in recipients of bone marrow transplants: Analysis of regulatory T-cell imbalances in GVHD. Blood. 1982;59:696.

  9. Giorgi J. Lymphocyte subset measurements: significance in clinical medicine. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:236-246.

  10. Haynes B. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:3-30.

  11. Jackson A, Warner N. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:226-235.

  12. Jondal M, Holm G, Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972;136:207-215.

  13. Kan E, Wang C, Wang L, Evans R. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983;131:536-539.

  14. Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:259-288.

  15. Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975;256:495.

  16. Lanier L, Le A, Phillips J, Warner N, Babcock G. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983;131:1789-1796.

  17. Ledbetter J, Evans R, Lipinski M, Cunningham-Rundles C, RA G, Herzenberg L. Evolutionary conservation of surface molecules that distinguish T-lymphocyte helper/inducer and T cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981;153:310-323.

  18. Mishell B, Shiigi S, Henry C, et al. Preparation of mouse cell suspensions. In: Mishell B, Shiigi S, eds. Selected Methods in Cellular Immunology. New York: WH Freeman and Co; 1980:16-17.

  19. Schroff R, Gale R, Fahey J. Regeneration of T-cell subpopulations after bone marrow transplantation: Cytomegalovirus infection and lymphoid subset imbalance. J Immunol. 1982;129:1926.

  20. Schroff R, Gottlieb M, Prince H, Chai L, Fahey J. Immunological studies of homosexual men with immunodeficiency and Kaposi’s sarcoma. Clin Immunol Immunopathol. 1983;27:300-314.

  21. Smolen J, Chused T, Leiserson W, Reeves J, Alling D, Steinberg A. Heterogeneity of immunoregulatory T-cell subsets in systemic lupus erythematosus: Correlation with clinical features. Am J Med. 1982;72:783-790.

  22. van Dongen J, Krissansen G, Wolvers-Tettero I, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988;71:603-612.

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BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗,提高传染性疾病诊断的质量和速度,推进新型药物和疫苗的研究与发现。公司于1897年在纽约成立,总部位于美国新泽西州的富兰克林湖,业务可分为BD医疗、BD诊断、BD生物科学三大类,生产销售包括医用耗材、实验室仪器、抗体、试剂、诊断等产品。

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