Description

Interleukin-10 (IL-10), (aka Cytokine Synthesis Inhibitory Factor CSIF), is an 18.5 kD protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of proinflammatory T cell mediated immunity.

Purity and Formulation: The rat IL-10 was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beer-Lambert Law. The endotoxin level is ≤ 0.1 ng per µg of IL-10.

Recombinant rat IL-10 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered Dulbecco"s PBS pH 7.2 and 1.0 mg/ml BSA carrier protein.

Suggested Companion Products

Preparation and Storage

This preparation contains no preservatives, thus it should be handled under aseptic conditions.Store product at -80°C prior to use or for long term storage of stock solutions.Rapidly thaw and quick-spin product prior to use.Avoid multiple freeze-thaws of product.Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less that 0.5 - 1.0 mg/ml carrier protein* such as human or bovine serum albumin, aliquoted and stored at -80°C. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

Product Notices

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.