BD Biosciences/CD15 FITC/332778,BD Biosciences,,BD Biosciences,bdbiosciences,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：BD Biosciences/CD15 FITC/332778

产品编号：332778

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： BD Biosciences

公司：BD Biosciences,Inc.

公司分类：

商品介绍

Product DetailsRecommended AssayReferences

Description

CD15 is intended for in vitro diagnostic use in the identification of cells expressing CD15 antigen, using a BD FACS™ brand flow cytometer.

The flow cytometer must be equipped to detect light scatter and the appropriate fluorescence, and be equipped with appropriate analysis software (such as BD CellQuest™ or BD LYSYS™ II software) for data acquisition and analysis. Refer to your instrument user’s guide for instructions.

Format

FormatFITC
Excitation SourceBlue 488 nm
Excitation Max494nm
Emission Max520nm

FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

Resources & Tools
Spectrum Viewer	Panel Designer	SpectrumViewer	Download MSDS	Download TDS	IFU	CFDA License	IFU

Preparation and Storage

The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes: Approved Guideline. Wayne, PA: Clinical and Laboratory Standards Institute; 2007. H42-A2.
Protection of Laboratory Workers from Occupationally Acquired Infections–Second Edition; Approved Guideline. Villanova, PA: National Committee for Clinical Laboratory Standards; 2001. NCCLS document M29-A2.
Andrews RG, Torok-Storb B, Bernstein ID. Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies. Blood. 1983;62:124-132.
Arber DA, Weiss LM. CD15: a review. Appl Immunohistochem. 1993;1:17-30.
Bernstein ID, Andrews RG, Cohen SF, McMaster BE. Normal and malignant human myelocytic and monocytic cells identified by monoclonal antibodies. J Immunol. 1982;128(2):876-881.
Bettelheim P, Lutz D, Majdic O, al. e. Cell lineage heterogeneity in blast crisis of chronic myeloid leukaemia. Br J Haematol. 1985;59:395-409.
Cantu-Rajnoldi A, Putti C, Saitta M, al. e. Coexpression of myeloid antigens in childhood acute lymphoblastic leukaemia: relationship with the stage of differentiation and clinical significance. Br J Haematol. 1991;79:40-43.
Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.
Drexler HG, Sagawa K, Menon M, Minowada J. Reactivity pattern of "myeloid monoclonal antibodies" with emphasis on MCS-2. Am J Pathol. 1986;10:17-23.
Hanjan SN, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identi es a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol. 1982;23:172-188.
Hsu SM, Jaffe ES. Leu-M1 and peanut agglutinin stain the neoplastic cells of Hodgkin"s Disease. Amer J Clin Path. 1984;82:29.
Jackson AL, Warner NL. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose NR, Friedman H, Fahey JL, eds. Manual of Clinical Laboratory Immunology. 3rd ed. Washington, DC: American Society for Microbiology; 1986:226-235.
Pinkus GS, Thomas P, Said JW. Leu-M1—A marker for Reed-Sternberg cells in Hodgkin"s disease: an immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 1985;119:244.
Rothe G, Schmitz G. Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies. Leukemia. 1996;10:877-895.
Skubitz K, Balke J, Ball E, et al. Report on the CD15 cluster workshop. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press Inc; 1989:800-805.
Stelzer GT, Marti G, Hurley A, McCoy P, Jr., Lovett EJ, Schwartz A. US-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry. 1997;30:214-230.
Van Voorhis WC, Steinman RM, Hair LS, et al. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J Exp Med. 1983;158:126-145.

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