BD Biosciences/Anti-Human CD45RA FITC/CD45RO PE/CD3 PerCP/CD8 APC/20 μL/340574,BD Biosciences,,BD Biosciences,bdbiosciences,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：BD Biosciences/Anti-Human CD45RA FITC/CD45RO PE/CD3 PerCP/CD8 APC/20 μL/340574

产品编号：340574

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场地：美国(厂家直采)

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商品介绍

Product DetailsRecommended AssayReferences

Description

The CD45RA antibody, clone L48, is derived from hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with low-buoyant– density human lymphocytes.

The CD45RO antibody, clone UCHL-1, is derived from hybridization of P3/NS-1/1- Ag4-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with an interleukin-2 (IL-2)–dependent human T-cell line.

The CD3 antibody, clone SK7, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

The CD8 antibody, clone SK1, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

The CD45RA antibody recognizes a 220-kilodalton (kDa) molecular weight isoform of the leucocyte common antigen (LCA). The CD45RA antigen is a member of the CD45 antigen family that also includes the CD45, CD45RB, and CD45RO antigens.

The CD45RO antibody, a member of the same CD45 family as CD45RA, recognizes a 180-kDa isoform of the LCA. The CD45 antigen is a protein tyrosine phosphatase.

The CD3 antibody recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).

The CD8 antibody recognizes an antigen expressed on the 32-kDa α subunit of a disulfide-linked bimolecular complex. The cytoplasmic domain of the α subunit of the CD8 antigen is associated with the protein tyrosine kinase p56^lck. The CD8 molecule interacts with class I major histocompatibility complex (MHC) molecules resulting in increased adhesion between the CD8⁺ T lymphocytes and the target cells. Binding of the CD8 molecule to class I MHC molecules enhances the activation of resting T lymphocytes.

Specificity	Clone	Format	Isotype	Entrez Gene ID
CD8	SK1	APC	IgG₁, κ	925
CD45RA	L48	FITC	IgG₁, κ	5788
CD45RO	UCHL1	PE	IgG_2a, κ	5788
CD3	SK7 (also known as Leu-4)	PerCP	IgG₁, κ	916

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Preparation and Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

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