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℡ 4000-520-616
BD Biosciences/CD45 FITC/CD14 PE/20 µL/340040
产品编号:340040
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
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品      牌: BD Biosciences
公      司:BD Biosciences,Inc.
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BD Biosciences/CD45 FITC/CD14 PE/20 µL/340040
商品介绍
Product DetailsRecommended AssayReferences

Description

BD Simultest™ Leucogate™ (CD45/CD14) is a two-color direct immunofluorescence reagent for establishing an optimal lymphocyte gate for immunophenotyping of erythrocyte-lysed whole blood (LWB). BD Simultest Leucogate is for in vitro diagnostic use with BD in vitro diagnostic reagents. See the appropriate BD in vitro diagnostic package insert for additional information.

Contents

SpecificityCloneFormatIsotypeEntrez Gene ID
CD452D1FITCIgG1, κ
CD14MφP9(also known as MφP-9)PEIgG2b, κ929
Preparation and Storage

• For in vitro diagnostic use.

• When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.

• The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.

• Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

• The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

  1. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes; Tentative Guideline. Villanova, PA: National Committee for Clinical Laboratory Standard; 1992. NCCLS document H42-T.

  2. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture–Fourth Edition; Approved Standard. Wayne, PA: National Committee for Clinical Laboratory Standards; 1998. NCCLS document H3-A4.

  3. Bernard A, Boumsell L, Hill C. Joint report of the First International Workshop on Human Leucocyte Differentiation Antigens by the investigators of the participating laboratories: M2 protocol. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman S, eds. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:82-108.

  4. Bernstein ID, Self S. Joint report of the myeloid section of the Second International Workshop on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1984:1-25.

  5. Beverley P. Production and use of monoclonal antibodies in transplantation immunology. In: Touraine J, Trager J, Betuel H, eds. Transplantation and Clinical Immunology XI. 1980:87-94.

  6. Cobbold S, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael A, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press Inc; 1986:788-803.

  7. Dimitriu-Bona A, Burmester G, Waters S, Winchester R. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983;130:145-152.

  8. Giorgi J. Lymphocyte subset measurements: significance in clinical medicine. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:236-246.

  9. Goyert S, Tesio L, Ashman L, et al. Report on the CD14 Cluster Workshop. In: Knapp W, Dörken B, Gilks WR, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:789-794.

  10. Herrmann F, Komischke B, Odenwald E, Ludwig W. Use of monoclonal antibodies as a diagnostic tool in human leukemia. I. Acute myeloid leukemia and acute phase of chronic myeloid leukemia. Blut. 1983;47:157-163.

  11. Jackson A, Warner N. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:226-235.

  12. Jackson A. Basic phenotyping of lymphocytes: selection and testing of reagents and interpretation of data. Clin Immunol Newslett. 1990;10:43-55.

  13. Jayaram Y, Hogg N. Surface expression of CD14 molecules on human neutrophils. In: Knapp W, Dörken B, Gilks WR, et al., eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:796-797.

  14. Loken M, Brosnan J, Bach B, Ault K. Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry. 1990;11:453-459.

  15. Rowe D, Beverley P. Characterisation of breast cancer infiltrates using monoclonal antibodies to human leucocyte antigens. Br J Cancer. 1984;49:149-159.

  16. Schwinzer R. Cluster report: CD45/CD45R. In: Knapp W, Dörken B, Gilks WR, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press Inc; 1989:628-634.

品牌介绍

BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗,提高传染性疾病诊断的质量和速度,推进新型药物和疫苗的研究与发现。公司于1897年在纽约成立,总部位于美国新泽西州的富兰克林湖,业务可分为BD医疗、BD诊断、BD生物科学三大类,生产销售包括医用耗材、实验室仪器、抗体、试剂、诊断等产品。

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